Cloned (Comment) | Organism |
---|---|
cDNA, from brain, expression in Escherichia coli M15/pRER4, sequencing, ORF is located on chromosome 22q12.3, genomic organization | Homo sapiens |
full-length cDNA, from brain, sequencing, ORF is located on chromosome 15.E1, genomic organization | Mus musculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0025 | - |
pyridoxal 5'-phosphate | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
0.0434 | - |
pyridoxine 5'-phosphate | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
0.0806 | - |
pyridoxamine 5'-phosphate | pH 7.4, 37°C, recombinant enzyme | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | requirement for a divalent cation, Mg2+ is most effective in catalyzing the dephosphorylation of pyridoxal 5-phosphate | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
31512 | - |
x * 31512, sequence calculation | Mus musculus |
31698 | - |
2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE | Homo sapiens |
32000 | - |
2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE | Homo sapiens |
60000 | - |
gel filtration, recombinant enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | vitamin B6 metabolism, catabolism of pyridoxal 5-phosphate | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q96GD0 | - |
- |
Mus musculus | P60487 | - |
- |
Purification (Comment) | Organism |
---|---|
9.6fold, recombinant enzyme | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | - |
Mus musculus | - |
brain | highly abundant in, most highly represented in all the regions of central nerve system except the spinal cord | Homo sapiens | - |
liver | major expression site additional to brain and testis | Homo sapiens | - |
additional information | mRNA is differentially expressed in a tissue-specific manner, tissue distribution | Homo sapiens | - |
testis | major expression site additional to brain and liver | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
1.4 | - |
pH 7.4, 37°C, hydrolysis of pyridoxal 5-phosphate, recombinant enzyme | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | vitamin B6 metabolism, catabolism of pyridoxal 5-phosphate | Homo sapiens | ? | - |
? | |
additional information | very low activity with with p-nitrophenyl phosphate | Homo sapiens | ? | - |
? | |
pyridoxal 5'-phosphate + H2O | - |
Mus musculus | pyridoxal + phosphate | - |
? | |
pyridoxal 5'-phosphate + H2O | highest specificity constant followed by pyridoxine 5-phosphate | Homo sapiens | pyridoxal + phosphate | - |
? | |
pyridoxamine 5'-phosphate + H2O | - |
Homo sapiens | pyridoxamine + phosphate | - |
? | |
pyridoxine 5'-phosphate + H2O | - |
Mus musculus | pyridoxine + phosphate | - |
? | |
pyridoxine 5'-phosphate + H2O | - |
Homo sapiens | pyridoxine + phosphate | - |
? | |
pyridoxine 5'-phosphate + H2O | second highest specificity constant after pyridoxal 5-phosphate | Homo sapiens | pyridoxine + phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 31512, sequence calculation | Mus musculus |
dimer | 2 * 31698, sequence calculation, 2 * 32000, recombinant enzyme, SDS-PAGE | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.45 | - |
pyridoxamine 5'-phosphate | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
1.25 | - |
pyridoxine 5'-phosphate | pH 7.4, 37°C, recombinant enzyme | Homo sapiens | |
1.52 | - |
pyridoxal 5'-phosphate | pH 7.4, 37°C, recombinant enzyme | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mus musculus | sequence calculation | - |
6 |
Homo sapiens | sequence calculation | - |
6 |